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Targeted Gene Finder & Functional Sequence Analyzer

🧬 DNA Sequence Analyzer

Targeted Gene Prediction and Mutation Impact Analysis

Overview

This project is a bioinformatics tool developed using Python that performs DNA sequence analysis, including ORF detection, mutation analysis, transcription, translation, and graphical visualization.

The system is built using Biopython and includes a user-friendly GUI for easy interaction.

Features

  • DNA → RNA transcription
  • RNA → Protein translation
  • Open Reading Frame (ORF) detection
  • Mutation detection and classification
  • Sequence alignment
  • GC content analysis
  • Graph visualization (GC content & ORF distribution)
  • GUI-based interface

Technologies Used

  • Python
  • Biopython
  • Tkinter
  • Matplotlib

📂 Project Structure

DNA_Analyzer/
│── utils.py     # Core logic
│── main.py      # CLI version
│── gui.py       # GUI version

▶How to Run

1. Install Dependencies

pip install biopython matplotlib

2. Run GUI Version (Recommended)

python gui.py

3. Run CLI Version

python main.py

Sample Input

Original DNA:

ATGAAAGGGTTTCCCAAAGGGTTTCCCAAAGGGTTTCCCAAAGGGTTTCCCAAAGGGTTTCCCAAAGGGTTTCCCAAAGGGTTTCCCTAA

Mutated DNA:

ATGAAAGGGTTTCCCAAAGGGTCTCCCAAAGGGTTTCCCAAAGGGTTTCCCAAAGGGTTTCCCAAAGGGTTTCCCAAAGGGTTTCCCTAA

Output

  • ORF positions and scores
  • Protein sequences
  • Mutation classification
  • Graphical plots

References

  • Biopython Documentation
  • Python Documentation
  • NCBI Resources

🏁 Conclusion

This project demonstrates how computational tools can be used to analyze biological sequences efficiently and visualize important genetic information.