Targeted Gene Finder & Functional Sequence Analyzer

🧬 DNA Sequence Analyzer

Targeted Gene Prediction and Mutation Impact Analysis

Overview

This project is a bioinformatics tool developed using Python that performs DNA sequence analysis, including ORF detection, mutation analysis, transcription, translation, and graphical visualization.

The system is built using Biopython and includes a user-friendly GUI for easy interaction.

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Features

• DNA → RNA transcription
• RNA → Protein translation
• Open Reading Frame (ORF) detection
• Mutation detection and classification
• Sequence alignment
• GC content analysis
• Graph visualization (GC content & ORF distribution)
• GUI-based interface

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Technologies Used

• Python
• Biopython
• Tkinter
• Matplotlib

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📂 Project Structure

DNA_Analyzer/
│── utils.py     # Core logic
│── main.py      # CLI version
│── gui.py       # GUI version

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▶How to Run

1. Install Dependencies

pip install biopython matplotlib

2. Run GUI Version (Recommended)

python gui.py

3. Run CLI Version

python main.py

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Sample Input

Original DNA:

ATGAAAGGGTTTCCCAAAGGGTTTCCCAAAGGGTTTCCCAAAGGGTTTCCCAAAGGGTTTCCCAAAGGGTTTCCCAAAGGGTTTCCCTAA

Mutated DNA:

ATGAAAGGGTTTCCCAAAGGGTCTCCCAAAGGGTTTCCCAAAGGGTTTCCCAAAGGGTTTCCCAAAGGGTTTCCCAAAGGGTTTCCCTAA

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Output

• ORF positions and scores
• Protein sequences
• Mutation classification
• Graphical plots

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References

• Biopython Documentation
• Python Documentation
• NCBI Resources

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🏁 Conclusion

This project demonstrates how computational tools can be used to analyze biological sequences efficiently and visualize important genetic information.

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